@Cell growth promotion of human skin fibroblasts by dermatan sulfate
released into wounds following injury remains uncertain. The aim of
the present study is to elucidate the growth enhancement and differential
production of various kinds of cell growth factors of human dermal diploid
fibreblasts HSF-T2 by exogeous dermatan sulfate in cell culture.
@We investigated the effects of exogenous dermatan sulfate addition
upon cell proliferation and the synthesis of growth factors by HSF-T2
cells. The HSF-T2 cells proliferated up to 6-fold higher ratio compared
with HSF-T2 cells alone in dose response manner to dermatan sulfate
concentrations ranged from 5 to 50 mg/ml. Furthermore, we examined the
same experiment in the case of dermatan sulfate concentrations present
in wound fluid from 5 to 80Κg/ml, it enhanced the proliferation of HSF-T2
having about 2.5-fold ratio compared with control.
@ We measured cell growth factors, such as HGF, bFGF, TGF-ΐ1, VEGF,
and PDGF-AA, in conditioned medium produced by HSF-T2 cells with dermatan
sulfate. Much or/and slightly higher growth factor values were obtained
in coculture with HSF-T2 and dermatan sulfate than in single cultures
of HSF-T2. Moreover, the cocentration of HGF in conditioned medium produced
by HSF-T2 was highest than the other factors. On the@other hand, EGF
and PDGF-AA could not detected in cultured medium in tested and control
@ Our results indicate that dermatan sulfate released during wound repair
are a potent mediator or director of fibroblast growth and that provide
the first suggestive evidence of mechanism of wound healing.